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Registros recuperados: 41 | |
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Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker. |
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041 |
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Junqueira,OM; Fonseca,LEC; Araújo,LF; Duarte,KF; Araújo,CS da S; Rodrigues,EAp. |
The experiment was conducted to evaluate the effect of feed restriction and different sodium levels in the diet on the performance and hematological values (erythrocyte, hemoglobin, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration and white blood cell) of broilers from 22 to 42 days of age. A completely randomized design was used in a 4 x 2 x 2 factorial (levels of sodium: 0.16%, 0.19%, 0.22% and 0.25%; with or without feed restriction; male and female), and two replicates of thirty broilers each. Sodium levels did not affect performance (p>0.05). Feed restriction resulted in increased feed intake and affected weight gain and feed conversion negatively (p<0.05). Better weight gain and feed conversion (p<0.05)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood; Broilers; Feed restriction; Nutrition; Sodium; Sodium bicarbonate. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200003 |
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Aride,P.H.R.; Oliveira,A.T.; Oliveira,A.M.; Ferreira,M.S.; Baptista,R.B.; Santos,S.M.; Pantoja-Lima,J.. |
ABSTRACT Tambaqui,Colossoma macropomum is a fish of primary importance in Brazilian aquaculture and in the Amazon region in particular. The aim of this work is to analyze the combined effects of physical training and levels of dietary cassava (Manihot esculenta) on the hematological parameters, food intake, conversion efficiency, growth ratio and swimming performance of this fish. A diet for tambaqui consisting of 30% cassava caused decreases in weight gain and specific growth rate compared with the control group. Diets containing 15% or 45% cassava did not affect these indices and did not cause hematological changes in tambaqui juveniles, indicating that some amount of cassava can be used as an alternative energy source for this important aquaculture... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Teleosts; Growth; Feed evaluation; Stress; Blood. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601697 |
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Ledic-Neto, Jerko; Universidade Federal de Santa Catarina; Dotta, Geovana; Universidade Federal de Santa Catarina; Garcia, Patrícia; Universidade Federal de Santa Catarina; Brum, Aline; Universidade Federal de Santa Catarina; Gonçalves, Eduardo Luis Tavares; Universidade Federal de Santa Catarina; Martins, Maurício Laterça; Universidade Federal de Santa Catarina. |
The objective of this study was to investigate the influence of dietary supplementation with propolis on hematology and number and area of melanomacrophage centers in spleen and kidney of tilapia. After acclimation, fish (24.7 ± 7.4 gmean weight) were distributed in 6 tanks 100 L, 6 fish per tank, in triplicate, at a temperature 24.0 ± 2.8oC, with two treatments: Fish fed 2% propolis supplemented diet and fish fed non-supplemented diet. To monitor the evolution of the effects, two samples were collected: half of the fish from each treatment were used after 15 days and the other half after 21, composing two feeding times. After each feeding time, blood, spleen and kidney were collected. After twenty one days feeding on 2% propolis supplemented diet, fish... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.06.03.00-0 fish; Blood; Kidney; Spleen; Hemosiderin Aquicultura. |
Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/22024 |
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Fock,R.A.; Vinolo,M.A.R.; Blatt,S.L.; Borelli,P.. |
The objectives of this study were to determine if protein-energy malnutrition (PEM) could affect the hematologic response to lipopolysaccharide (LPS), the interleukin-1β (IL-1β) production, leukocyte migration, and blood leukocyte expression of CD11a/CD18. Two-month-old male Swiss mice were submitted to PEM (N = 30) with a low-protein diet (14 days) containing 4% protein, compared to 20% protein in the control group (N = 30). The total cellularity of blood, bone marrow, spleen, and bronchoalveolar lavage evaluated after the LPS stimulus indicated reduced number of total cells in all compartments studied and different kinetics of migration in malnourished animals. The in vitro migration assay showed reduced capacity of migration after the LPS stimulus in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood; Bone marrow; Spleen; Interleukin-1β; Lipopolysaccharide; Malnutrition. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200009 |
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Tavares-Dias,Marcos; Oliveira-Júnior,Antônio Aderson; Marcon,Jaydione Luiz. |
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon turtles; Blood; Leukocytes; Podocnemis expansa; Thrombocytes. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020 |
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MARIANO,WAGNER S.; AZEVEDO,SAULO B.; GOMES,FRANCISCO L.; LIMA,LIANA B.D.; MORON,SANDRO E.; TAVARES-DIAS,MARCOS. |
Abstract: This study investigated the physiological effects of a pesticide based on Bacillus thuringiensis (Dipel-WP®) added in the water and diet of Piaractus mesopotamicus during 24 and 48 h. It was added 0.13 g of de B. thuringiensis per kg of commercial feed; and for the fish subjected to the biopesticide in the water of the tanks, it was added 0.13 g/L of the biopesticide. Plasma levels of sodium, chloride, potassium, cholesterol, glucose, triglycerides, cortisol, total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST), hematocrit, hemoglobin, erythrocytes number, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), number of thrombocytes and leukocytes were determined. Cortisol, lactate, glucose,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemistry; Blood; Farm fish; Freshwater fish. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300612 |
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Costa e Silva,Luiz Fernando; Marcondes,Marcos Inácio; Valadares Filho,Sebastião de Campos; Ribeiro,André Mauric Frossard; Prados,Laura Franco; Nascimento,Fernando Barros; Gionbelli,Mateus Pies; Santos,Ronald Matos dos. |
This study was conducted to develop equations to predict chemical composition of head, limbs, hide and blood in cattle. A database containing 335 animals from 10 trials, with 221 Nellore, 38 Nellore-Simmental and 76 Nellore-Angus (96 steers, 118 heifers and 121 bulls) animals was used. Models were constructed to estimate water, ether extract (EE), crude protein (CP), ash and macrominerals (calcium, phosphorus, magnesium, sodium and potassium) in the non-carcass parts of cattle. A stepwise procedure was conducted to determine the most significant variables within each model. Subsequently, a random coefficient model was used to construct the equation using studies as random effect, and sex and breed as fixed effects. The visceral fat was the most important... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood; Carcass; Ether extract; Hide; Organs and viscera; Protein. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000800015 |
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ISHIKAWA, M. M.; PÁDUA, S. B.; SATAKE, F.; PIETRO, P. S. de; HISANO, H.. |
O monitoramento do estado de saúde dos peixes é imprescindível para o manejo sanitário da produção, seja de grande ou pequeno porte. A hematologia clínica é uma ferramenta que permite a realização de diagnóstico de patologias e pode atuar como um indicador prognóstico das condições patológicas, especialmente quando se considera as alterações morfológicas nas células sanguíneas (SATAKE et al., 2009). Estas avaliações permitem identificar a resposta dos peixes, quando doentes, de maneira rápida, prática e de baixo custo |
Tipo: Circular Técnica (INFOTECA-E) |
Palavras-chave: Manejo sanitário; Harvest.; Colheita; Peixe; Sangue.; Blood; Fish.. |
Ano: 2010 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/874164 |
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Faria, Leonardo Avanzzi Nunes; UEM; Barbosa, Orlando Rus; UEM; Zeoula, Lucia Maria; UEM; Aguiar, Silvia Cristina de; UEM; Prado, Rodolpho Martin do; UEM; Bertolini, Dennis Armando; UEM. |
Avaliou-se o comportamento, as respostas sanguíneas de animais confinados recebendo dieta com produto à base de própolis (LLOS). Os tratamentos foram: controle (CON) e dois produtos à base de própolis (LLOSC1) e (LLOSC1+). Mediram-se os comportamentos: comendo (COM), bebendo água (BEB), andando (AND), ruminando em pé (RUEMP), ruminando deitado (RUDET), em pé (EMP) e deitado (DET). Maior tempo em COM, BEB, AND, RUEMP e EMP ocorreu de manhã (MA) e à tarde (TA), e em RUDET e DET à noite (NO) e madrugada (MD). Animais no Lado 1 do confinamento permaneceram mais tempo andando na MA e à NO, sem diferenças para MD, e mais tempo EMP para os do Lado 1 na MA, T e MD, e igual à NO. Cortisol, Glicose e Ureia não foram influenciados pelos tratamentos. Não houve efeito... |
Tipo: Pesquisa de campo |
Palavras-chave: 5.04.01.00-9 comportamento animal; Confinamento; Própolis; Sangue animal behavior; Feedlot; Propolis; Blood. |
Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10671 |
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Oliveira, Wagner Dias Coelho de; Silva, Tairon Pannunzio Dias e; Bezerra, Leílson Rocha; Araújo, Marcos Jácome de; Edvan, Ricardo Loiola; Lopes Oliveira, Ronaldo. |
The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemistry; Blood; Ketone bodies; Metabolites; Physiology. biochemistry; Blood; Ketone bodies; Metabolites; Physiology.. |
Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/49022 |
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Denadai,JC; Ducatti,C; Pezzato,AC; Carrijo,AS; Caldara,FR; Oliveira,RP. |
This paper aimed at evaluating the influence of diets containing different isotopic values of carbon-13 turnover on the half-life of egg (yolk + albumen), yolk and albumen individually, and blood of poultry using delta13C isotopic variation. Commercial layers fed four experimental isocaloric and isonitrogenous diets (RC4, RC3, RMC4 and RMC3) containing different isotopic values, during an experimental period of 56 days. Turnover of the studied tissues was influenced by the experimental diets. Blood and albumen were more influenced by dietary treatments as compared to egg and yolk. The RMC3 diet induced better performance (better feed intake and higher egg production) due faster rate of carbon substitution than the RC4 diet, and lower half-life for egg... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Albumen; Carbon-13; Yolk; Blood; Egg; Comercial laying hens. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400008 |
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Dailidavičienė,J.; Budreckienė,R.; Gružauskas,R.; Kerzienė,S.; Andrulevičiūtė,V.; Sinkevičienė,I.. |
ABSTRACT The aim of the study was to determine the effect of Saccharomyces cerevisiae, multienzyme composition supplementation on milk yield, quality, blood biochemical parameters of Lithuanian-Black-and-White cows. For this reason 28 cows were divided into four groups (three experimental and one control) each with seven cows fed balanced ration (control group) and following experimental groups: addition of 40g supplement of live yeast with organic selenium (group A); 40g supplement of live yeast with aromatic additives (group B); and 0.2g supplement of multienzyme composition (group C) during a 90 days period. The study showed that milk yield was 2.64%, 1.75%, 1.4% higher in groups A, B, C respectively, comparing with the control group. The milk SCC in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cow; Probiotic; Yeast; Milk yield; Blood. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300939 |
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Barbosa,Julicelly Gomes; Costa,Roberto Germano; Medeiros,Ariosvaldo Nunes de; Queiroga,Rita de Cássia Ramos do Egypto; Batista,Ângela Maria Vieira; Medeiros,Geovergue Rodrigues de; Beltrão Filho,Edvaldo Mesquita. |
The objective of this study was to evaluate intake, apparent digestibility, ingestive behavior and blood parameters of milking goats fed cactus pear (Opuntia ficus indica) and increasing urea levels in substitution to soybean meal. Ten multiparous Alpine goats with average weight of 31±6.0 kg were used and distributed into (5 × 5) Latin squares and assessed during five 17-day periods, receiving five treatments with the following substitution levels: 0; 5; 10; 15 and 20 g/kg of urea. Neutral detergent fiber (NDF), non-fiber carbohydrates, ether extract, organic matter and water intake values showed differences, whith average values ranging from 1.03 to 1.13; 0.12 to 0.14; 1.76 to 2.10 kg/day and 0.77 to 1.48 kg/day, respectively. The NDF and crude protein... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood; DM intake; Milking goats; Rumination. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000700022 |
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Registros recuperados: 41 | |
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